Résumé:
This work deals with phytochemical and pharmacological study of two species Salvia
phlomoides Asso. from Lamiaceae family and Ferula vesceritensis Coss. Et Dur. from the
Apiaceae family.
The separation and purification by chromatographical methods of chloroform and ethyl
acetate extract of Salvia phlomoides led to the isolation of nine compounds including four
triterpenoids from which three are new, four flavonoids, one phenolic acid, and three
flavonoids from the ethyl acetate extract of Ferula vesceritensis. The structural determination
of the isolated compounds was established on the basis of spectral analysis IR; ESI-HR-MS,
1D and 2D NMR (1H NMR, 13C NMR, DEPT-135, HSQC, HMBC, COSY and NOESY), and
comparison with literature data.
Evaluation of the cytotoxicity of the new triterpenoids showed a selective inhibitory
effect against the panel of human tumor cell lines NCI-60 of the National Cancer Institute of
the United States of America. Evaluation of the antioxidant activity by different methods of
the ethyl acetate and n-butanol extracts of Salvia phlomoides and ethyl acetate extract of
Ferula vesceritensis indicates an important antioxidant capacity.
The GC and GC-MS analysis of the essential oils of the two species showed that the
essential oil of Salvia phlomoides (studied for the first time) was mainly represented by
Hexahydrofarnesyl acetone, Dibutyle phtalate, Phytol, α-Humulene, Caryophyllene Oxyde
and β-Caryophyllene, and major components of the essential oil of Ferula vesceritensis
(South) were Shyobunol, δ-Cadinene, ϒ-Cadinene, Isoledene and Dauca-5,8-diene, while for
Ferula vesceritensis (East), D-Carvone and Rotundifolone were found as major components
for the first time in the genus Ferula.
