الخلاصة:
The present work consisted on a phytochemical and biological study of the species Pituranthos battandieri Maire, Algerian variety from the Apiaceae family, endemic to the Algerian and Moroccan Sahara. To the best of our knowledge this species has never been studied before. This reason and the endemism of this rare species encouraged us to undertake this work in which we also reported the chemical composition of the essential oil of its aerial parts performed by GC and GC/MS
analyses.
Our experimentation allowed the separation and purification of 23 compounds
through the use of various chromatographic techniques (CCM, CC). All these
structures have been elucidated by the combination of spectroscopic methods mainly:
(UV-Vis), 1D NMR (1H, 13C, DEPT), and 2D NMR (COSY, HSQC, HMBC,
NOESY), We also used high resolution mass spectrometry methods (HR-EI-MS and
HR-ESI-MS) and comparison with literature data. Among the isolated compounds, 13
coumarins from which 5 were new, 4 flavonoids, 2 steroids, 3 phenolic compounds
and 1 reduced sugar.
In addition, a biological valorization consisting in the evaluation of the
antioxidant activity was carried out on the n-BuOH extract of the aerial parts of
Pituranthos battandieri Maire. This study showed that this species has a biological
efficiency. In this study we also carried out an antibacterial assay against some
pathogenic organisms, the microbiological results showed that this butanolic extract
did not show antibacterial activity.
The GC-FID and GC/MS analyses of the essential oil of Pituranthos
battandieri Maire showed that this oil is mainly composed of myrcene (8.9%), α-
phellandrene (17.5%), α-terpinene (14%), o-cymene (11.6%), β-phellandrene (3.5%),
cis-linalol oxide (furanide) (5.9%) and trans-β-caryophyllene (3.4%).
