Résumé:
The main objective of our work is phytochemical studies and evaluation
of secondary metabolite products extracted from Thymelaeaceae family
which is rich in biological active phenolic compounds. So, we choose
Thymelaea microphylla, which grows in Algeria. In addition to another plant
from the same family; Gnidia somalensis growing in Yemen.
Our study also, devoted to the evaluation and effectiveness of the plant
extracts and pure compounds against cancer cells. We used the ELISA and
xCELLigence assay technics for determining the inhibition of fractions and
pure compounds in vitro, against HeLa cells (human uterus carcinoma) and
C6 cells (rat brain tumor). Which have shown positive results, especially for
fractions that have a high activity compared with the reference compound. In
addition, we have got a significant results by testing the pure compounds
(Prestegane B, daphnoretin and trans-tiliroside) which gave a positive results.
On the other hand, we were able to isolated 10 compounds from T.
microphylla:
• Two lignan lactones.
• One bicoumarin.
• Four flavonoids.
• Two spiro lactones.
• One new alkaloid (microphybenzimidazole).
Also, we have separated 12 phenolic compounds from the plant Gnidia
somalensis: 6 polysubstituted cinnamic acid derivatives and 6 flavonoids.
We used different chromatographic methods for separation and
purification (Column chromatography CC, thin layer chromatography TLC),
and spectroscopic tools for structure identification (HPLC LC-MS, HPLCTOF), ID (Tf -NMR, 13C- NMR and DEPT) and 2D (HMQC, HMBC and
COSY).
