دور المستخلص البيتانولي لنباتين من جنس ) (Genista et Limoniumفي الوقاية من سمية مبيد الكلوربيريفوس

Abstract

Although mostly known as an organophosphorus pesticides (OPs) Chlorpyrifos (CPF) can react also as a pro-oxidant resulting in the generation of (ROS) (Reactive oxygen species). The purpose of this study was to evaluate the protective effect of Genista ulicina Spach and Limonium pruinosum extract Towards the oxidative stress which implied (involved) in the toxicity of this pesticide. Our result indicated that administration of CPF at a dose (10mg/kg/BW) for 10 days by oral gavage induced a significant increase in MDA level in all tissues, organs; transaminase enzymes ALAT, ASAT; Triglycerides, cholesterol, Creatinine and Uric Acid; on the other hand the activity of antioxidant enzyme including GPx, CAT and GSH levels were significantly decreased. Histopathological observations also correlated with the biochemical parameters was shown. Also, the protective effects of Buthanolic extract of Genista ulicina Spach and Limonium pruinosum (150mg/kg/BW) alone or in combination with CPF were investigated. Co-administration of plants extract with CPF or alone in male rats decreased MDA levels, normalized CAT and GPx activity as well as GSH content and restore some biochemical parameters by suppressing CPF stress. Furthermore Milder pathological alterations were observed. Overall, the protective effects of plants extract on CPF induced toxicity may be due to its potent antioxidative properties Antioxidant activities of the extracts were studied in vitro. The inhibition of the formation of malondialdehyde (MDA) and the scavenging of DPPH radicals were assayed. The total phenolic and flavonoid contents of the extracts were determined. The experimental results show that the extracts had antioxidant activity in vitro. The extracts showed a high antioxidant effect, especially scavenging of DPPH anions and inhibition of lipid peroxidation. That extracts can inhibit membrane LPO and free radical formation due to its free radical scavenging ability. The biological and antioxidant effects exhibited by these extracts could be related to an overall effect of the phenolic compounds present in this extract .Leading to ensure a protective role in attenuating the oxidative stress generated by CPF.