Abstract:
Although mostly known as an organophosphorus pesticides (OPs)
Chlorpyrifos (CPF) can react also as a pro-oxidant resulting in the generation
of (ROS) (Reactive oxygen species).
The purpose of this study was to evaluate the protective effect of Genista
ulicina Spach and Limonium pruinosum extract Towards the oxidative stress
which implied (involved) in the toxicity of this pesticide.
Our result indicated that administration of CPF at a dose (10mg/kg/BW)
for 10 days by oral gavage induced a significant increase in MDA level in all
tissues, organs; transaminase enzymes ALAT, ASAT; Triglycerides,
cholesterol, Creatinine and Uric Acid; on the other hand the activity of
antioxidant enzyme including GPx, CAT and GSH levels were significantly
decreased. Histopathological observations also correlated with the
biochemical parameters was shown.
Also, the protective effects of Buthanolic extract of Genista ulicina Spach
and Limonium pruinosum (150mg/kg/BW) alone or in combination with CPF
were investigated. Co-administration of plants extract with CPF or alone in
male rats decreased MDA levels, normalized CAT and GPx activity as well as
GSH content and restore some biochemical parameters by suppressing CPF
stress. Furthermore Milder pathological alterations were observed. Overall,
the protective effects of plants extract on CPF induced toxicity may be due to
its potent antioxidative properties
Antioxidant activities of the extracts were studied in vitro. The inhibition
of the formation of malondialdehyde (MDA) and the scavenging of DPPH
radicals were assayed. The total phenolic and flavonoid contents of the
extracts were determined. The experimental results show that the extracts had
antioxidant activity in vitro. The extracts showed a high antioxidant effect,
especially scavenging of DPPH anions and inhibition of lipid peroxidation.
That extracts can inhibit membrane LPO and free radical formation due to
its free radical scavenging ability. The biological and antioxidant effects
exhibited by these extracts could be related to an overall effect of the phenolic
compounds present in this extract .Leading to ensure a protective role in
attenuating the oxidative stress generated by CPF.
