L’effet protecteur de la plante Linaria tingitana (Scrophulariaceae) sur le dysfonctionnement mito chondrial et lysosomal hépato cytaire induit par l’acide valproïque chez le rat

Abstract

This investigation is interested to study the mo dulator effect of Linaria tingitana in rats treated by the valproic acid (VPA). The in vitro studies (DPPH ◦, FRAP, β-carotene, Chelation, NO ◦) showed that Linaria tingitana is endowed with an antioxidant property. The antiinflammatory studies (albumin denaturation, HRBC, NO scavenger, oedema and peritonitis led by the carrageenan, the rise of MPO and the infiltration of the PN) confirmed that Linaria tingitana has a beneficial effect in this sense. The in vivo studies made to rats handled by the VPA revealed that the n-BuOH extract acts by a multitude of mechanisms: conservation of the hepatic function, the modulation of the steatoses, the neutralization of the oxidative stress and the reduction of the inflammation hepatocyte elucidated by the decrease of the activity MPO. Antirrhides molecules isolated of this plant were ineffective at the level of the cell line HepG2 treated with the VPA. The mitochondrial dysfunction led by the VPA is expressed by the decrease of the RCR accompanied by a decrease of the activity of the complex I. The n-BuOH extract as well as the isolated molecules managed to reduce the mitochondrial H2O2 and to modulate the antioxidant mitochondrial and lysosomal systems. The conservation of the integrity lysosomal membrane was more pronounced by the n-BuOH extract compared to the vitamin E. The various modulator ways of L. tingitana delayed the appearance of the complications linked to the mitochondrial dysfunction, lysosomal, such necrosis. Probably this is due to the diversified wealth of this plant in term of polyphenols and irridoids revealed by phytochemical screening and the analysis of the LC-MS and in the synergetic effect of the molecules that it contains