Résumé:
In this study, Lactiplantibacillus plantarum R10 isolated from Bouhezza
was evaluated for their probiotic properties, bacteriocin production and safety profile using Genomics
analyses.
Objectives: The present work aims to study the whole genome sequence of R10 strain isolated
from Bouhezza to characterize the bacteriocin producing gene and evaluation of its probiotic properties.
Methods: In the present study, Lactiplantibacillus plantarum named R10 isolated from an
Algerian traditional goatskin bag cheese named Bouhezza was investigated for their probiotic
haracteristics using genomics analyses. The genome of the isolate was sequenced, and bioinformatic
analyses were conducted to identify probiotic-related genes in addition to the screening of virulence
and antibioresistance genes, to assess their safety.
Results and discussion: Whole genome sequencing results demonstrate the presence of various
genes involved in gastrointestinal stress tolerance, adhesion to host epithelial cells, indicating bacterial
adaptation of strains to the gastrointestinal environment. We also detected genes involved in the
antimicrobial activity (i.e. bacteriocin). No virulence or antibioresistant determinantwere found in the
genome sequence. The strain R10 fulfils all the qualities of being a probiotic and the genome insights
have given more information into the molecular machinery potentially involved in the probiotic effects.
Conclusion: These results suggest that Lactiplantibacillus plantarum R10 strain could be
considered as promising probiotic candidate. Therefore, technological performances should be explored
in order to promote their application in cheeses and other functional foods as a bio-protective or starter
culture
