Assays In Vitro of the biological control by using three species of Trichoderma against various species of Fusarium Agent of Fusarium

Abstract

Three isolates of Trichoderma are isolated from the ground of different regions in Algeria. The antagonist effect of these isolates has been studied on seven isolates of Fusarium , agent of Fusarium Head Blight, according to the method of direct confrontation and to distance. The results obtained revealed that the isolates of Tichoderma were able to inhibit the mycelial growth of Fusarium of more than 65% compared to the witness, and this after four days of incubation at 25 0C. In addition, in the sixth day the Trichoderma invades the colonies of Fusarium on which it wetspored itself, thus revealing its high myco-parasitic capacity. The obtained results of the indirect confrontation show a slowdown of the mycelial growth of Fusarium strains induced by Trichodermasp with a percentage reached 43% compared to the witnesses. This study conclude that the antagonistic strains can exert an inhibitory effect on the pathogenic colonies development