Abstract:
The aim of this work is to estimate the Total phenolic and flavonoid content and determine the
antioxidant activities of Silene gallica. The total phenolic and flavonoid contents were
investigated using Folin-Ciocalteu reagent assay (FCR) and the aluminium nitrate .The
antioxidant activity was evaluated through different methods: DPPH, ABTS and CUPRAC. The
EtOAc extract of S.gallica presented the higher content of polyphenols ( 204.441 ± 3.95µg
GAE/ml ) and flavonoids (102.998±1.35µg QE/ml ) followed by the BuOH extract (110.247 ±
2.50 µg GAE/ml) ,(84.37±2.36 µg QE/ml). For DPPH test, the maximum scavenging activity
was found in EtOAc extract (IC50 value: 42.36±0.14 µg/mL),at variance in the ABTS method
the BuOH extract exhibited highest activity (IC50 value: 23.92 ±1.86 µg/mL). In the CUPRAC
method, the EtOAc extract indicated higher activity (A0.50value: 28.27 ±0.91 µg/mL)
