The total phenolic and flavonoid contents and antioxydant effect of Sillene gallica

Abstract

The aim of this work is to estimate the Total phenolic and flavonoid content and determine the antioxidant activities of Silene gallica. The total phenolic and flavonoid contents were investigated using Folin-Ciocalteu reagent assay (FCR) and the aluminium nitrate .The antioxidant activity was evaluated through different methods: DPPH, ABTS and CUPRAC. The EtOAc extract of S.gallica presented the higher content of polyphenols ( 204.441 ± 3.95µg GAE/ml ) and flavonoids (102.998±1.35µg QE/ml ) followed by the BuOH extract (110.247 ± 2.50 µg GAE/ml) ,(84.37±2.36 µg QE/ml). For DPPH test, the maximum scavenging activity was found in EtOAc extract (IC50 value: 42.36±0.14 µg/mL),at variance in the ABTS method the BuOH extract exhibited highest activity (IC50 value: 23.92 ±1.86 µg/mL). In the CUPRAC method, the EtOAc extract indicated higher activity (A0.50value: 28.27 ±0.91 µg/mL)