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Etude phytochimique et pharmacologique de deux plantes sahariennes

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dc.contributor.author Djemam, Nihed
dc.contributor.author Benayache, Samir
dc.date.accessioned 2022-05-25T08:28:09Z
dc.date.available 2022-05-25T08:28:09Z
dc.date.issued 2020-11-18
dc.identifier.uri http://depot.umc.edu.dz/handle/123456789/8239
dc.description.abstract This work is devoted to the phytochemical, biological and pharmacological study of two endemic Saharan species; Helianthemum lippii (Cistaceae) and Cymbopogon schoenanthus (Poaceae). The extraction of the two species followed by the chromatographic separations (CC, CCM and HPLC semi-preparative), led to isolate and identify using spectroscopic methods (1D and 2D NMR) 6 compounds among which two sterols, as well as four sesquiterpenes: Stigmasterol, β- Sitosterol, (1S,4aa)-4Methylene-6-(1-hydroxy-&-methyethyl)-8a-b-methyl-decahydroxydronaphtalene-Ab-ol 1S, 7S, 10S, eudesm-4(14)-en-1α-11α-diol(hydroxy-β-eudesmol), 4α, 11-dihydroxy-7-epimereudesmandiol (7-epi-cryptomeridiol), 4β, 7β, 11-enantioeudesmantriol (Pterodontriol), 4β,6β,10α-trihydroxy guaiane sesquiterpène (Teuclatriol). Chromatographic analysis of the essential oils of Cymbopogon schoenanthus showed that the hydrocarbons represented 26.7% while the sesquiterpene fraction reached 40%. The HPLC-TOF / MS analysis of the AcOEt and n-BuOH extracts of the two species suggested the presence of 32 phenolic acids and flavonoids which were present in different proportions The efficacy of inhibiting DNA damage and the evaluation of the anticancer activity carried out on the EtOAc and n-BuOH extracts of the two species demonstrated a particularity observed in Helianthemum lippii on the antioxidant effect and thus the antiproliferative effect. The pharmacological study was carried out on the effect of the extracts on the distal colon of Wistar rats; which have shown a marked relaxing effect on the isolated distal colon of the rat which could represent an underlying mechanism for the traditional use of these plants for gastrointestinal conditions. Further studies will help us understand the metabolites responsible for the effect and mechanism involved.
dc.language.iso fr
dc.publisher Université Frères Mentouri - Constantine 1
dc.subject Chimie: Chimie organique
dc.subject Chimie organique : Analyses physicochimiques, contrôle de la qualité et synthèse de substances bioactives
dc.subject Helianthemum lippii
dc.subject Cymbopogon schoenanthus
dc.subject séparations chromatographiques
dc.subject méthodes spectroscopiques
dc.subject huiles essentielles
dc.subject analyse HPLC-TOF/MS
dc.subject activité antioxydante
dc.subject activité anticancéreuse
dc.subject colon distal du rat
dc.subject effet relaxant
dc.subject chromatographic separations
dc.subject spectroscopic methods
dc.subject essential oils
dc.subject HPLC-TOF / MS analysis
dc.subject antioxidant activity
dc.subject antioxidant activity
dc.subject anticancer activity
dc.subject rat distal colon
dc.subject relaxing effect
dc.subject التحليل الكرماتوغرافي
dc.subject طرق التحليل الطيفي
dc.subject الزيوت الأساسية
dc.subject النشاط المضادة للأكسدة
dc.subject النشاط المضاد للسرطان
dc.subject القولون النازل
dc.subject تحليل HPLC-TOF/MS
dc.subject تأثير الاسترخاء
dc.title Etude phytochimique et pharmacologique de deux plantes sahariennes
dc.title Helianthemum Lippii et Cymbopogon schoenanthus.
dc.type Thesis

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