Etude phytochimique et évaluation des activités biologiques d'Abies numidica de lannoy et de cerdrus atlantica (EndI) Manetti (Pinaceae)

Abstract

Separations and purifications by chromatographic methods allowed us to isolate 8 abietanediterpene compounds from the cones of Abies numidica de Lannoy and 21 compounds from the cones of Cedrus atlantica (Endl) Manetti including 3 tocotrienolic acids from wich one is new, 3 flavonoids from which one is new, 3 neolignanes and 12 abietane-diterpenes. The structural determination of the isolated compounds from these two Pinaceae was based on the use of 1D and 2D NMR techniques (1H, 13C, COSY HH, HSQC, HMBC and NOESY), mass spectrometry (ESI-MS and HRESI-MS ) and on comparison with literature data. Evaluation of the antioxidant activity of the fractions derived from the VLC of the hydromethanolic extract of the cones of A. numidica showed a higher activity of the F60:40 fraction in the ABTS + test, followed by fractions F40:60 and F80:20. Furthermore, the F60:40 fraction of the hydromethanolic extract of the cones of A. numidica was the best inhibitor of the enzyme acetylcholinesterase whereas the F80:20 fraction showed the highest activity against butyrylcholinesterase. The extracts of the two studied plants and the isolated compounds showed a good antimicrobial activity against a large spectrum of Gram-positive bacteria and on the tested fungi (Candida).