Etude phytochimique et évaluation des activités biologiques de deux plantes du genre Sedum

Abstract

The present works concern two plants of Sedum genus (Crassulaceae) : S. caeruleum and S. pubescens. The extraction and chromatographic separations (CC, TLC) of the chloroform, ethylacetate, and n-butanol extracts of S. caeruleum permitted the isolation of 6 products which were identified by the use of spectroscopic methods (UV, NMR 1D and 2D), as : 1- Ursolic acid 2- Daucosterol (β-sitosterol-3-O-β-D-glucopyranoside) 3- β-sitosterol-3-O-β-D-galactopyranoside 4- Apigenin 5- Apigetrin 6- Apiin The LC-ESI-MS/MS of the n-butanol fraction of S. caeruleum permitted the identification of 23 compounds from which 11 phenolic acids and 12 flavonoid glycosides. The results of the biological activities of S. caeruleum and S. pubescens were as follows: - The n-butanol fractions of S. caeruleum and S. pubescens showed the best antiradical activity with the DPPH test. - The chloroform fraction of S. caeruleum and the n-butanol fraction of S. pubescens showed the best activity with the CUPRAC test. - The n-butanol fraction of S. caeruleum showed the best activity with the ion chelate test. - The n-butanol fraction of S. caeruleum and the n-butanol fraction of S. pubescens exhibited the best activity with the β-carotene test. - The n-butanol fraction of S. caeruleum inhibited the activity of the butyrylcholinesterase. - The chloroform fraction de S. caeruleum showed an excellent anti-inflammatory activity which was higher that the standard’s (ibuprofen) - The chloroform fraction de S. caeruleum inhibited mildly the growth of the tested bacterial strains.