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dc.contributor.authorBensmira Soumia
dc.contributor.authorMeraihi, Zahia
dc.date.accessioned2016-11-14T13:04:51Z
dc.date.issued2006-06-12
dc.identifierhttp://bu.umc.edu.dz/md/index.php?lvl=notice_display&id=1348
dc.identifier1348
dc.identifier20110104u u u0frey50 ba
dc.identifier.urihttp://hdl.handle.net/123456789/7885
dc.description88 f.
dc.description.abstractThe microscopic study of the moulds isolated from Sebkhas of Biskra area allowed, to identify 7 different kinds from 10 potentially cellulolytic strains: Aspergillus, Penicillium, Geotrichum, Altarnaria, Cladosporium, Altarnaria and Trichoderma. The fermentation of the 10 strains selected and cultivated on a medium containing date scrap (under product of pheonicical industry) as a substrate of fermentation, allowed to select hypercellulolytic strains with a cellulasic activity of 775 U. The strain (R3), isolated from the ground soil of palm plantations of Biskra corresponds to the Trichoderma kind. This train is retained for the kinetic study of the biomass, the cellulasic activity, sugar, proteins like that of the pH. This study reveals that the synthesis of the enzyme reaches its maximum after 60 hours of fermentation during the stationary phase of the growth. A partial purification with ammonia sulphate 40 % of saturetion reduced the content of proteins of 78.4 % and increased the specific activity to 94.35 % which reached the degree of purification to 17.70. The enzyme was found very thermostable with a half-life of 170 , 140, 120 and 50 munites at 60, 70, 80 and 90°C respectively. The corresponding temperature for the maximum cellulolytic activity was 55°C whereas the optimim pH was 4.8.
dc.languagefre
dc.languagefr-fr
dc.publisherUniversité Mentouri Constantine
dc.subjectBiologie
dc.subjectDéchets de dattes
dc.subjectIdentification
dc.subjectThermostabilité
dc.subjectCellulase
dc.subjectIsolement
dc.subjectMilieux extremes
dc.subjectMoisissures
dc.subjectIsolation
dc.subjectextreme medium
dc.subjectfungi
dc.subjectdate scrap
dc.subjectcellulase
dc.subjectthermostability
dc.subjectعزل
dc.subjectتعريف
dc.subjectوسط اقصى
dc.subjectوسط اقصى
dc.subjectبقايا التمور
dc.subjectسلولاز
dc.subjectمقاومة الحرارة
dc.titleIsolement et caractérisation de souches fongiques de milieux extremes (sol et sebkha de la région de Biskra) productrices de cellulase themostable à interet industriel
dc.typeThesis
dc.coverage01 Disponible à la salle de recherche 02 Disponibles au magazin de la B.U.C.


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