"Étude des infections causées par les actinomycètes aérobie autres que les mycobactéries dans la région de Constantine "

Abstract

"Actinomycetes are Gram-stain-positive. Ubiquitous, they live as saprophyte in the soil. Although they are rarely encountered in clinical practice, they have great potential and cause serious human infections. Forty-two strains of aerobic actinomycetes (except mycobacteria) were isolated from 3682 clinical specimens patients from Benbadis Hospital of Constantine. The diversity and identification of some representative isolates were determined by a polyphasic approach (phenotypic, genotypic and phylogenetic informations). Phylogenetic analysis of the isolates allowed to classify them in the phylum of Actinobacteria, to the families of Nocadiopsaceae, Pseudonocardiaceae, Micromonosporaceae, Tsukamurellaceae, and Thermomonosporaceae. They are affiliated to seven different genera, Nocardiopsis (53.33%), Pseudonocardia (13.33%), Murinocardiopsis (6.66%), Tsukamurella (6.66%), Actinomadura (6.66), Micromonospora (6.66) et Saccharopolyspora (6.66%). Approach by proteomics analysis of mass spectrometry MALDI-TOF has been validated and optimized for the identification and classification of bacteria of the genus Tsukamurella. Through the MLST method and the amplification of four genes (lepA, rpoA, rpo B and ftsZ) and the technique of mass spectrometry we were able to conclude that isolate 1534 is probably a new species of Tsukamurella. Furthermore, sequencing of the complete genome of this isolate was done by two sequencing techniques, the shotgun method and 3-kb paired-end."