Etude de l’effet de l’interaction du molybdene avec l’azote chez les fabacees cultivees en milieu salin

Abstract

In Algeria grain legumes are considered as an important nourishing food, they are very rich in proteins. Molybdenum has an important role in several biological processes in plants, that is why our research focuses on three legumes species (bean, chickpea and lens) submitted to salt stress and in presence of molybdenum and nitrogen. Combination of molybdenum and nitrogen in salt stress (3g/l of NaCl) condition, leads to an increase in fresh shoot biomass and chlorophyll (a+b). Molybdenum and nitrogen stimulate synthesis of total proteins in bean but in roots of chickpea and lens, salt stress causes their increase. In bean and chickpea shoots, xanthine dehydrogenase activity is stimulated by both molybdenum and nitrogen. Aldehyde oxidase activity is stimulated in bean shoots by adding molybdenum and nitrogen, and in lens roots in salt stress condition. Sulfite oxidase has a decreasing activity which decreases with time and is affected by salt stress. Nitrate reductase activity increases in time and is affected by salt stress. The activity of the molybdenum cofactor decreases because of salt stress except for lens which increases. For bean, sulphite oxidase and xanthine dehydrogenase genes have a role in adaptation to salt stress condition. The expression of mARC chickpea gene and mARC2 bean gene is stimulated in condition of salt stress which gives them a role to salt stress adaptation