Abstract:
Ten bacterial strains were isolated from clinical specimens provided by the bacteriology
laboratory (DAKSI). They were identified by standard microbiological methods (biochemical
gallery). Among the ten strains studied, eight strains belonging to the Enterobacteriaceae and
two strains are among the Gram-negative bacilli non-fermentative (Pseudomonas). These
strains were identified presumptively by their biochemical characteristics, susceptibility or
resistance to antibiotics, and total nitrogen measured by the Kjeldahl method. A phylogenetic
profile was produced by phenetic distance methods, using NJ algorithm and sequence analysis
of 16S DNA. The analysis of topologies obtained was used to determine the place of the
biochemical phenotype in establishing phylogenetic relationships.
