Le sapin d'Algérie (Abies numidica de Lannoy ex Carrière): étude phytochimique des métabolites secondaires et évaluation in vitro, in vivo et in silico des activités biologiques de ses composés.

Abstract

This study aims to enhance the virtues of an Algerian endemic plant which is the Algerian fir ""Abies numidica de Lannoy ex Carrière"" by a phytochemical characterization and an evaluation of the biological activities of its leaves. The phytochemical study revealed the main secondary metabolites found in particular were polyphenols (phenolic acids, flavonoids and tannins). LCMS / MS Analysis of the polyphenolic in extracts revealed the richness of the leaves of the Algerian fir in phenolic compounds, 12 molecules were identified in the AE fraction and 8 compounds were determined in the n-BuOH extract. GC-MS analysis showed the presence of 29 terpene compounds. The evaluation of the antioxidant power in vitro by colorimetric methods (DPPH •, ABTS • +, reducing power, CUPRAC and phenanthroline) showed that the AE and n-BuOH extracts endowed with a high antioxidant power. The antimicrobial effect of the medicinal plant varies depending on the nature of the strain and the extract tested. The extracts exhibited a significant cytotoxicity against Artemia salina lethality. In vivo, the AE and n-BuOH extract from the leaves of A. numidca showed no mortality at the concentration of 2000 mg / kg. Furthermore, biochemical parameters confirmed the presence of liver damage, by increasing the concentration of liver transaminases. The in silico toxicity study confirmed that the extracts were not toxic. The present study confirmed the effectiveness of the plant and provided measurable biological proof of its antioxidant, enzyme inhibitory and antibacterial power.