Abstract:
This work focused on an essential oil (EO) of a plant species, Laurus
nobilis (L. nobilis) which belongs to the Lauraceae family, one of the families most used in traditional
therapy.
Objectives: The main objectives of this study were phytochemical screening and biological
activities; antioxidant, antibacterial and mainly antibiofilm; of the EO of L. nobilis harvested from the
region of Constantine.
Methods: The phytochemical screening was based on specific tests for each metabolite. Besides,
the antioxidant activity of the EO was tested using two methods, DPPH and FRAP. The antibacterial
activity by the well diffusion method of the EO was additionally tested on four strains; S. aureus, B.
cereus, E. coli, and P. aeruginosa. The antibiofilm activity of the EO was also conducted on S. aureus.
Results and discussion: It was highlighted the presence of the main bioactive metabolites like
polyphenols, flavonoids, tannins, and EO which were the most dominant. In addition, the EO recorded
lower IC50 and EC50 (0.14 mg/ml and 0.13 mg/ml, respectively) having the most relevant antioxidant
potency. As well as, very interesting inhibition zone diameters were recorded. The obtained results
revealed that Gram + bacteria are the most sensitive to EO; where the highest inhibition value was
recorded on S. aureus (32±0.23 mm) at a concentration of 100%. The EO also exhibited a very high
antibiofilm activity on S. aureus with a percentage reduction of 76.1% at 100%; moderately high to that
of chloramphenicol (70.3%).
Conclusion: These results can be considered as a starting point for the use of L. nobilis EO in the
field of health as a natural ATB against S. aureus biofilm infections to avoid the successive use of
synthetic ATBs, and therefore the reduction of bacterial resistance
