Abstract:
Our research purpose to study the effect of the metabolic by-products of the Aspergillus fumigatus
fungus on the germination of some types of lentil seeds. Purification has been achieved with
organic solvents prior to bar chromatography using an aluminium oxide bar AL2O3, fallowed by thin
layer chromatography transported to Silica gel F254 plates. After drying, values of the circulation
rate (0.937-0.051)Rf for every resulting spotwere determined. Spots occur with different colours
under UV radiation with short wave 254 nm and 365 nm for long waves and likewise in regular light.
It allowed determining ten close in their chemical structure.With the study of the impact of different
concentrations from secondary metabolic issues secreted by Aspergillus fumigatus on lentil seed
germination: sp. I (Syrie), sp. II Large blonde (chili), sp. III Esculata lens (verte). The experiment
was designed in sterilized sandy fields inside Petri. The minimal germination percentage rate for
the lentil seeds (60.65%) was recorded at concentration 1 mg for Large blonde ( chili ) and
Escualata lens ( verte ), whereas Syrie amounted to 70% at the same concentration. Furthermore,
this rate was recorded for Esculata lens( verte) at concentration 100 µg and for LBC type at
concentration 1 µg. That means resistance capacity in Syrie exceeds 100, 10 times the ELV and
LBC capacity respectively. It should be pointed out that sensitivity in the lentil types started at
concentration 0.25 ng and germination percentage was 92.95 and 95% for ELV, LBS, Syrie
respectively
