Abstract:
Secondary metabolites produced by plants for their self-defense mechanisms have
been implicated in human health and therapeutic properties for most plants such as
Artemisia genus. This genus, widespread over the world with up to 500 species, belongs to the
Asteraceae family, locally named “Dgouft”. In these study three extractions methods were
used for evaluation antioxidant activities and analyzed quantitatively artemisinin in Artemisia
campestris leaves using High Performance Liquide Chromatography (HPLC). Decoction,
maceration and Ultrasound-assisted extractions (UAE) were widely used for the extraction
secondary metabolites such as essential oils, flavonoids and total antioxidant activity. The
extract antioxidant activities were evaluated using hydrogen atoms transfer methods (DPPH,
total antioxidant capacity, and reducing power assays) and single electron transfer (ABTS and
cupric reducing antioxidant capacity assays). The contents of artemisinin in A.campestris
leaves extract were over 0.6%. The crud aqueous and methanolic extracts exhibited an
antioxidant potential (14,63±0,84 and 36,82±0,55 μg mL−1). Our findings revealed good
antioxidant and antimicrobial activities of Artemisia campestris extracts
