Antioxidant, α-amylase inhibitor and GC/MS analysis of chlorophormic fraction of Astragalus membranaceus

Abstract

Currently, the potential utilization of natural plant-derived extracts for medicinal and therapeutic purposes has increased remarkably. In this investigation potential inhibitors of α amylase, one of the key regulatory enzymes in diabetes, and in vitro antioxidant activities using hydrogen atoms transfer methods DPPH, β-carotene, ABTS and cupric reducing antioxidant capacity assays were characterized from the chloroformic fraction of aerial part of Astragalus membranaceus. For viable use of the extract, qualitative analysis of phytochemicals and their identification was carried out by gas chromatography-mass spectroscopy. The powerful inhibitor of alpha amylase was (IC50 =36.73+- 4.02 µg/mL). The better antioxidant activity is observed in β-carotene which is estimated at (IC50 = 131.78 3.71 µg/mL). The presence of 9, 12, 15 octadecatrienoic acid was identified by GC-MS and appeared as the most dominant constituent in chlorophormic extract