Étude phytochimique et évaluation des activités antioxydante, antibactérienne et cytotoxique de deux plantes du genre Phagnalon (Asteraceae)
Cherchar ép Saadi, Hanene
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Our phytochemical and biological works concerned the aerial parts of two species belonging to Asteraeae family : Phagnalon saxatile (L) Cass. and Phagnalon sordidum L. Several chromatographic methods allowed us to isolate ten products from the n-butanol and ethyl acetate extracts of Phagnalon saxatile from which one is new, and twenty one products from Phagnalon sordidum. Structures were determined by different spectral methods: nuclear magnetic resonance with different applications (NMR 1H, NMR 13C, COSY, HSQC and HMBC), the mass spectrometry, UV, acid hydrolysis as well as comparison with literature data. 4 methods were used to evaluate the antioxidant activity of Phagnalon sordidum and Phagnalon saxatile. The latter showed a good activity by the use of 4 methods: DPPH, ABTS, CUPRAC and superoxide radical scavenging activity by alkaline DMSO whereas Phagnalon sordidum exhibited a moderate activity with DPPH and CUPRAC tests. Furthermore, petroleum ether and chloroform extracts from Phagnalon saxatile showed moderate antibacterial activity against the tested strains. The isolated products (1-O- β-D-glucopyranosyl-1,4-dihydroxy-2-((E) 2-oxo-3-butenyl) benzene, 1-O-β-glucopyranosyl- 2(3’,3’-diméthylallyl) hydroquinone and Potassium 4-hydroxy-3-methoxybenzoique acide methyl ester-5-sulfate) from Phagnalon saxatile showed a moderate cytotoxic activity by the use of MTT method.
- Doctorat (Chimie)