L’effet protecteur des extraits de la plante Centaurea sphaerocephala L. vis-à-vis de la toxicité induite par l’acide valproïque
Lahneche, Amina Maya
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This work is devoted to study the protective effect of n-butanol extract of Centaurea sphaerocephala (c. sphaerocephala) against the impact toxic of valproic acid (VPA) associated with oxidative stress on different tissues in male Wistar albino’s rats. For that, n-Butanol extract of C. sphaerocephala (50mg/kg and 100mg/kg) and VPA (300mg/kg) were administered by gavage for 14days. At the end of the experiment or in the 15th day, animals were scarified, dissected and different organs (liver, kidney, brain and testicular) were removed, homogenized and used for determination of lipid peroxidation and antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx) and the level of reduced glutathione (GSH)). A small species of these organs were used for histopathological examination. The dysfunction caused by the VPA is expressed by a significant increase of lipid peroxidation reported by a determination of the level of tissues MDA and a significant decrease in GSH level and activity of enzymes antioxidant enzymes activities (catalase and GPx). This accompanied by a changes in biochemical parameters characterized especially by an increase in the concentration of creatinine, blood sugar, cholesterol levels, triglycerides and Aminotransferase (AST, ALT) which are released once that liver cells damaged and their membrane perforated. As well, the histological’ results showed clear alterations in the histoarchitecture of the different tissues studied: liver, kidney and testicular. Indeed, the pretreatment of rats by n-butanolic extract of C. sphaerocephala (50mg/kg, 100mg/kg) and Vit E (100mg/kg) restored the biochemical parameters to their normal values and also improved the antioxidant parameters as well as the histological appearance of the different tissues studied. n-butanol extract of this plant has a protective effect action against the oxidative stress resulting from the treatment of rats by the VPA due to its antioxidant effect. The in vitro Antioxidant tests (scavenging the radical DPPH, inhibition of LPO,) of the extracts the different phases (ethyl acetate and n-butanol) of C. Sphaerocephala indicated that various extracts have the role of free radical’s scavengers. In addition, these activities are strongly correlated with the levels of flavonoids and total phenols which are relatively high. The anticancer test was carried out using (HeLa) cell line by analyzing the different doses of the n-butanol extract of C. sphaerocephala plant. The results indicate that the extract has an anti-cancer effect concentrations dependent.