|dc.description.abstract||In this work, we studied an accession of durum wheat grown in Algeria with two varieties:
leucomeulon and reichenbachi, on which phenological, morpho-physiological and molecular
characteristics were measured. The different results obtained made it possible to identify several
distinct groups within this accession.
The phenological and morpho-physiological parameters revealed a certain intra- and inter-varietal
variability. Thus, most genotypes of the reichenbachi variety proved to be more effective for
morphological and physiological parameters, whereas many genotypes of the leucomelon variety
gave a better yield.
Across the accession, molecular markers RAPD and ISSR analyzed 50 selected genotypes belonging
to the two varieties.
For the RAPD markers, on 2 primers used, 2 showed monomorphic bands and a high percentage of
polymorphism of 92.10%. Moreover, the dendrogram divided the different genotypes into two large
distinct clusters of similarity located between 71, 2 and 100%.
Of these 50 genotypes, 20 were retained and analyzed using 2 other primers which generated 19
polymorphic markers with a high percentage of polymorphism equal to 95%.
For the ISSR markers, the primers used for the 50 selected genotypes were found to be polymorphic
with a total of 32 reproducible bands of which 22 were polymorphic (68.7%). The dendrogram of
molecular markers ISSR revealed two distinct heterogeneous groups of similarity slightly less than
Furthermore, the hierarchical classification of the two RAPD and ISSR markers of the 50 genotypes
studied made it possible to distinguish between two main groups with a similarity between 33 and
The evaluation of genetic diversity using the two molecular markers RAPD and ISSR used is more
discriminating for RAPD, which showed an important inter-varietal genetic polymorphism.We found
that the distribution of the 50 genotypes tested was based on their botanical variety.
In conclusion, the study of the phenological, morpho-physiological, yield and molecular parameters
analyzed by the various techniques and methods revealed certain inter varietal variability, but within
each of the two varieties, the genotypes were genetically close.||fr_FR