Recherche et détermination structurale de métabolites secondaires de deux plantes Algériennes de la famille des lamiaceae
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This work deals with the structural determination and biological evaluation of natural substances isolated from two species of the Lamiaceae family (Salvia aegyptiaca and Saccocalyx satureioides). These family and the genus Salvia are known for their rich antioxidant compounds including polyphenols and flavonoids. The phytochemical study of the aerial parts of these species allowed after extraction, separation and purification the isolation and identification of nineteen compounds from Saccocalyx satureioides and fourteen compounds from Salvia aegyptiaca .These compounds were identified by the combination of spectroscopic methods including : UV-visible absorption spectroscopy, high resolution HR-MS mass spectrometry, 1H and 13C-NMR and two-dimensional experiments (COSY, NOESY, HSQC, and HMBC) The isolated compounds were : A flavonol : Quercetin (14). Fifteen flavones : 7-methyl- sudachitin (8), Sideritiflavone (17), Luteolin (25), Apigenin (27), Salvigenin(28), 4',7-dimethylapigenin (29), 5-hydroxy 3', 4', 6,7- tetraméthoxyflavone(30), IsoIscutellarein-7-O-allosyl (1→2) glucoside (11),Isoscutellarein-7-O-[ β-D-allopyranosyl-(1→2)]-6’’-O-acetyl- β-D-glucopyranoside (12), Isoscutellarein-7-O-[6”’-O-acetyl- β-D-allopyranosyl-(1→2)]- β-Dglucopyranoside (13) , Isoscutellarein -7-O-[6‴-O -acetyl-β-D-allopyranosyl (1→2) ]- 6″-O-acetyl-β-D-glucopyranoside. (15), Apigenin- 7-O-(6’’-trans-p-coumaroyl- β -Dglucopyranoside (16), Luteolin 7-O glucoside (21), Apigenine 7-O glucoside (22), Diosmin (23). Sixteen phenolic compounds : Piceol(1), Vanillin (2), Ferulic aldehyde (3), 3,3’-bis (3,4- dihydro-4-hydroxy-6,8-dimethoxy-2H-1-benzopyran) (4), 3,3’-bis (3,4-dihydro-4- hydroxy-6-methoxy-2H-1-benzopyran) (5), Dimethylcaffeic acid (6), Balanophonin (7), Caffeic acid (9), p-coumaric acid (10), 4-methoxyphenol, Salicylic acid, Caffeic acide (18), Vanillic acid (19), Syringic acid (20), Rosmarinic acid (24), Methyl rosmarinate (26). A furocoumarin : 6`,7`-dihydroxybergamottin(31)The study of the chemical composition of essential oil of Salvia aegyptiaca species growing in Algeria was characterized by a high level of sesquiterpene fraction and a moderate level of monoterpene components. β-caryophyllene (10.2%), Selina-4,11-diene (9.7%), bornyl acetate (8.5%), β-gurjunene (7.6%), germacrene D (7.0 %) and β-caryophyllene oxide (6.2%) were the major components of this essential oil. This work led to the publications of two articles subject entitled “ On-line screening and identification of antioxidant phenolic compounds of Saccocalyx satureioides Coss. et Dur.” and “ Chemical composition of the Essential oil of Salvia aegyptiaca” . A third paper is in preparation and will be devoted to the phytochemical and biological study of Salvia aegyptiaca.
- Doctorat (Chimie)