http://depot.umc.edu.dz/handle/123456789/14450 Tue, 02 Jun 2026 08:29:38 GMT 2026-06-02T08:29:38Z http://depot.umc.edu.dz/handle/123456789/14718 HO-07: Prevalence and resistance profile of Acinetobacter baumannii isolated in the microbiology laboratory of the Regional Military University-Hospital of Constantine (HMRUC SEKHRI-ARAFA, Nedjoua; BOUZERAIB, Latifa; MEZIANI, Meriem; CHENTLI, Amira Acinetobacter baumannii, an opportunistic pathogen, emerged in the early 20th century. Over time, it has evolved by deploying antibiotic resistance mechanisms, making it a striking example of the evolution of microbial resistance. A. baumannii is identified as one of the nosocomial pathogens of greatest concern. In fact, in France, Acinetobacter baumannii is responsible for more than 90% of clinical isolates. Objectives: The fundamental objective of this study is to isolate strains of Acinetobacter baumannii from samples received from different departments of a hospital in Constantine, identify them and then determine their resistance profiles to various antibiotics. Methods: A comparative study (retrospective and prospective) was carried out on a sample of 86 strains of the Acinetobacter genus, including in particular 46 strains of the Acinetobacter baumannii species. These samples were collected between 2019 and 2023 from different samples, the majority of which were received from the intensive care unit (61.62%). The strains were isolated and identified by conventional methods in the microbiology laboratory. The resistance profile was established according to the CLCI recommendations. Results and discussion: All bacteria present significant resistance to the majority of antibiotics such as β-lactams, aminoglycosides and fluoroquinolones, however, they remain sensitive to colistin with a percentage of 97.8%. These results obtained support the multiresistance nature of Acinetobacter baumannii. Conclusion: Acinetobacter baumannii has become a global threat and poses enormous therapeutic problems for treating carrier patients. It is therefore essential to strengthen surveillance and apply good hospital hygiene practices as well as the rational use of antibiotics Thu, 05 Oct 2023 00:00:00 GMT http://depot.umc.edu.dz/handle/123456789/14718 2023-10-05T00:00:00Z http://depot.umc.edu.dz/handle/123456789/14717 HO-06: A comparative study between Argas persicus found in eastern Algeria and China RAHMANI, Amira; LAATAMNA, AbdElkarim; YU, Zhijun; LAFRI, Ismail; HERRADA, Zakaria4; CHITIMIA, DOBLER Lidia; BAKKES Deon, K Argas persicus (the fowl tick) is a soft tick, often found in and around the nesting areas of birds, particularly in poultry farms with a worldwide distribution. Objectives: In the current study, we performed morphological and molecular analyses to identify ticks collected in poultry farms from Algeria and East China. Methods: Ticks were collected from farms sites including walls, nests, cracks and crevices. All these sites were searched for finding of all ticks stages including nymph, larvae and adult. All the collected tick specimens were identified morphologically by microscope and then confirmed by molecular and phylogenetic analysis. Results and discussion: In total 30 ticks from poultry in Algeria, comprising 5 larvae, 12 nymphs, 12 adults, and 14 ticks comprising 12 Larvae and 2 adults from Gansu province in China were morphologically identified as A.persicus. Molecular analysis of 30 ticks from Algeria and the 14 Chinese samples based on PCR, sequencing, and phylogenetic analysis of the gene 16S rRNA confirmed morphological results. However, through phylogenetic analysis, it was revealed that all Algerian samples, along with two Chinese samples, were classified under Argas persicus sensu stricto (s.s.). Meanwhile, the remaining Chinese samples were identified as part of Argas persicus sensu lato (s.l.), constituting a distinct lineage due to their genetic divergence. Conclusion: The current research affirms the presence of A. persicus s.s. in both Algeria and China. Additionally, it presents new molecular data regarding a separate Chinese lineage of A.persicus Thu, 05 Oct 2023 00:00:00 GMT http://depot.umc.edu.dz/handle/123456789/14717 2023-10-05T00:00:00Z http://depot.umc.edu.dz/handle/123456789/14716 HO-05 : Activité anti-hémolytique des extraits des fruits de Pistacia Lentiscus MOULAOUI, Kenza; ZAIDI, Sid Ali; AZZI, Hassine; MOUHOUBI, Yacine; ATMANI, Djebbar Les biotechnologies appliquées à la santé concernent la santé humaine, que ce soit pour la prévention, la thérapie ou le diagnostic ; rester en bonne santé le plus longtemps possible est devenu la nouvelle idéologie de l’Homme occidental. Le stress oxydant est une circonstance anormale que traversent parfois nos cellules ou un de nos tissus lorsqu’ils sont soumis à une production, de radicaux libres oxygénés qui dépasse leurs capacités antioxydantes. De nombreuses affections humaines ou animales incluent donc un stress oxydant, local ou général, dans leur pathogenèse au même titre que l’inflammation, le vieillissement et le cancer à laquelle il est souvent associé. Dans ce contexte le présent travail porte sur l’étude de l’activité antioxydante de l’extrait methanolique d’une plante médicinale ; Pistacia lentiscus appartenant à la famille des anacardiacées, connue sous le nom de tidekt. L’activité antioxydante in vitro a été évaluée en utilisant le test de l’activité scavenger du radical libre DPPH et ABTS°+ ; l’extrait methanolique présente un meilleur effet anti-radicalaire avec une IC50 de 24,21± 0,14 (µg/ml) et de 1,541 ± 0,36 (µg/ml) respectivement. Ensuite un test vis-à-vis d’une agression oxydante des érythrocytes par deux agents ; le NaCl et la chaleur. Le test de cytotoxicité réalisé à différentes concentrations a montré que l’extrait methanolique représente un faible effet toxique. Le test anti hémolytique indique que l’extrait méthanolique des fruits de Pistacia lentiscus exerce un effet protecteur sur la membrane érythrocytaire contre le stress oxydant induit par hypotonie et le stress thermique. En conclusion, nous pouvons dire ainsi que l’extrait methanolique de Pistacia lentiscus est doté d’un pouvoir antioxydant et exprime un effet stabilisateur de la membrane du globule rouge, donc cette plante peut être une source très importante dans le domaine thérapeutique et pharmacologique Thu, 05 Oct 2023 00:00:00 GMT http://depot.umc.edu.dz/handle/123456789/14716 2023-10-05T00:00:00Z http://depot.umc.edu.dz/handle/123456789/14715 HO-04: Efficacy of Catechin, Quercetin and Gallic Acid On Oxidative Stress Induced By Etoposide On Human Red Blood Cells MAMERI, Amal; BENSALEM, Sihem; ADEL, Khadidja; BOUCHELKIA, Nasma; MOKRANE, IGUER-OUADAC; BOURNINE, Lamine Cancer progression, as well as the side effects of chemotherapy, have been associated with an imbalance between ROS and the antioxidant defense system. Chemotherapeutic agents used in the treatment of cancer are known to increase levels of ROS, free radicals and lipid peroxidation. Oxidative stress considered as a crucial mechanism in cell injury attack and subsequent peroxidative . As a result, the appearance of often irreversible cell and tissue toxicity and loss of the integrity of cell membranes. Thus, antioxidant compounds are seen as a promising remedy to neutralize ROS and ensure the protection of tissues against toxicities that could suppress the development of oxidative stress and cancers. This study aimed to evaluate the cytoprotective effect of three antioxidants, namely CAT (catechin), Q (quercetin), and GA (gallic acid), against oxidative stress (OS) induced by etoposide. Human red blood cells (RBCs) and hemoglobin (Hb) were pretreated with these antioxidants and then exposed to etoposide. Several measurements were performed to assess the protective effects. The inhibition of RBCs membrane cytotoxicity induced by etoposide by measuring cell turbidity, Hb release, methemoglobin (metHb) generation, and intracellular Hb content. The inhibition of Hb oxidation using CAT, Q, and GA was also evaluated by measuring Hb levels. Furthermore, this study explore the inhibition of membrane lipid peroxidation by these antioxidants to mitigate oxidative stress induced by VP16.The results indicated that at a concentration of 1 mM, CAT, Q, and GA enhanced cytoprotection against OS induced by VP16. Pretreatment with Q protected human RBCs against VP16 cytotoxicity, resulting in high cellular concentration (1.068±0.021). GA also improved the cytoprotective effect against VP16. Similarly, Hb release significantly decreased in RBCs exposed to VP16 when pretreated with CAT, GA, and Q with Hb rates about (0.233±0.04), (0.675±0.061) and (1.376±0.016), respectively.Moreover, the antioxidants Q, GA, and CAT exerted a highly significant inhibition of metHb generation against VP16. The inhibition rates were 63.15%, 33.43%, and 20.68% for Q, GA, and CAT, respectively. The intracellular Hb content was about (2.020±0.009) Q, (1.242±0.022) GA and (0.911±0.007) CAT, it was significantly higher in RBCs pretreated with these antioxidants, indicating significant cytoprotection against VP16. CAT, Q, and GA also protected Hb molecules against VP16-induced damage, as evidenced by high Hb levels.Furthermore, CAT, Q, and GA exhibited inhibition of lipid peroxidation against VP16, resulting in low levels of malondialdehyde (MDA) ranging from (0.002±0.017) to (0.054±0.002), which is a marker of lipid peroxidation. In conclusion, the three antioxidants (CAT, Q, and GA) demonstrated the ability to inhibit oxidative stress induced in human RBCs by VP16, a chemotherapy agent. They exerted a strong protective effect on RBC membranes, as well as on Hb molecules, potentially mitigating the side effects of chemotherapy-induced oxidative stress Tue, 05 Sep 2023 00:00:00 GMT http://depot.umc.edu.dz/handle/123456789/14715 2023-09-05T00:00:00Z