Depot institutionnel de universite Freres mentouri Constantine 1The DSpace digital repository system captures, stores, indexes, preserves, and distributes digital research material.http://depot.umc.edu.dz:802024-03-19T08:27:48Z2024-03-19T08:27:48ZIn vitro and In vivo Efficiency of Trichoderma harzianum against Phoma and Glocladium Soft Rot Occurred on Tomato Fruits (Lycopersicon esculentum)Hamitou, MokhtarDehimat, Laidhttp://depot.umc.edu.dz/handle/123456789/144412024-01-17T10:11:36Z2015-04-08T00:00:00ZIn vitro and In vivo Efficiency of Trichoderma harzianum against Phoma and Glocladium Soft Rot Occurred on Tomato Fruits (Lycopersicon esculentum)
Hamitou, Mokhtar; Dehimat, Laid
The present investigation aims to evaluate the in vitro and in vivo ability of
T.harzianum to control the Phoma and Glocladium soft rot, that occurred on
tomato fruits (Lycopersicon esculentum). Phoma sp. and Glocladium sp. were
isolated from infected tomato fruits, which were brought from Oum-elbouaghi
market, and identified in laboratory of microbiology, university of Oum-elbouaghi
(Algeria).One isolate of T.harzianum / Hypocrea lixii was brought from the
same laboratory. The results of direct confrontation (in vitro) of T.harzianum
against Phoma sp. and Glocladium sp. on PDA medium, showed that a different
inhibition in the mycelia growth of the tested fungus. That inhibition was equal in
the fourth day of the experiment to 39.58 % and 25.92% in Phoma sp. and in
Glocladium sp. respectively. The microscopic observations of mycelia showed that
the mycelia of T.harzianum was capable of overgrowing and degrading
mycelia and chlamydospores of Phoma sp., coiled around the mycelia of Phoma
sp. and Glocladium sp. However, it did not show any growth of the tested fungus
when re-planting a disk from the interaction hyphal area between T.harzianum and
Phoma sp. or Glocladium sp. from dual cultures, while T.harzianum grew alone in
plates. In vivo screening and after 7 days of incubation T.harzianum showed an
antagonistic activity against the tested fungus on tomato fruits, with inhibition
equal 71.43% and 100%, in Phoma sp. and in Glocladium s.p respectively,
compared with controls. Beside we found after cutting the superficial layer of the
tested tomato fruits, that the treated fruits with T.harzianum stayed saints,
compared with control, when Glocladium rot infected their deep tissues. This strain
of T.harzianum may offer potential for biological control of tomato Phoma and
Glocladium soft rot
2015-04-08T00:00:00ZThe antagonism between Trichoderma viride and other pathogenic fungal strains in Zea maysBouziane, ZDehimat, LAbdel aziz, WBenabdelkader, MKacem chaouche, Nhttp://depot.umc.edu.dz/handle/123456789/144402024-01-17T09:04:42Z2011-02-04T00:00:00ZThe antagonism between Trichoderma viride and other pathogenic fungal strains in Zea mays
Bouziane, Z; Dehimat, L; Abdel aziz, W; Benabdelkader, M; Kacem chaouche, N
Fungi such as Trichoderma and Gliocladium associated with parasitic behavior manifested by a
coil around the hyphae of fungi filaments. This study showed the antagonistic effect of
Trichoderma viride against the different fungal isolates infecting the plant Zea mays. The strain
of Trichoderma viride was isolated from Jijel soil character by humid climate. By different organs
(roots, stems and leaves) of plant Zea mays have been isolated 28 fungal strains belonging to 17
genus: Absidia, Alternaria, Aspergillus, Botrytis, Cladosporium, Epicoccum, Fusarium,
Geotrichum, Melanconium, Monileilla, Penicillium, Phoma, Pythium, Scopulariopsis, Scytalidium,
Trichoderma and Ulocladium. The test of direct confrontation between Trichoderma viride and
fungal isolates was made on PDA. The competitive action of Trichoderma viride on the pathogen
in the presence or absence of zone of inhibition was seen in this test. The growth rate of fungal
isolates and Trichoderma viride has been determined. Trichoderma viride reached the
confluence of the Petri dish four days after sowing, so that different fungal isolates occupy a
surface of 29% to Fusarium roseum, 13% for Epicoccum sp2, 44% for Epicoccum sp3, 21% for
Monileilla sp, 15% for Absidia sp and 6% for Trichoderma sp2 which corresponds to an inhibition
of mycelia growth of fungal isolates tested
2011-02-04T00:00:00ZINFLUENCE OF SECONDARY METABOLIC PRODUCTS OF ASPERGILLUS FUMIGATUS ON THE GERMINATION RATE OF SOME LENTIL TYPESDEHIMAT, LBOUZIANE, ZKACEM CHAOUCHE, NBELAIDI, AABDELAZIZ, WHARZALLAH, Dhttp://depot.umc.edu.dz/handle/123456789/144392024-01-17T08:38:06Z2009-06-29T00:00:00ZINFLUENCE OF SECONDARY METABOLIC PRODUCTS OF ASPERGILLUS FUMIGATUS ON THE GERMINATION RATE OF SOME LENTIL TYPES
DEHIMAT, L; BOUZIANE, Z; KACEM CHAOUCHE, N; BELAIDI, A; ABDELAZIZ, W; HARZALLAH, D
L’objectif de cette étude est d’étudier l’effet des produits métaboliques secondaires de Aspergillus
fumigatus sur la germination de quelques variérés de lentille. La technique a été réalisée en utilisant
des solvants organiques, puis une chromatographie ascendante avec une colonne Al2O3. En fin, une
chromatographie couches minces sur plaques de silice F254. Les rapports frontaux (Rf : 0.05 – 0.937)
ont été déterminés après séchage. Sous lumière UV (courte =254 nm et longue = 365 nm) et sous
lumière ordinaire, les taches ont donné différentes couleurs. Dix composés ont donc été déterminés.
L’expérience a été menée en boites de Pétri sur milieux sablés afin de tester l’effet des concentrations
des métabolites secondaires sur les différentes variétés de lentilles (Lentille (Syrie) spI, Lentille
(Large Bonde Chili) spII et Lentille (Esculanta Lens Verte)spIII). Le pourcentage (60.65%) le plus
faible (taux de germination) a été observé dans le cas de la variété Large Bonde Chili (LBC) et
Escualata lens avec une concentration de 1 mg. Cependant, un taux de 70% a été atteint (pour la
même concentration) dans le cas de la variété Syrienne. Les variétés Esculata lens( verte) et LBC ont
montré le même taux, mais à la concentration de 100mg. Il faut retenir que la sensibilité des variétés
aux métabolites s’est prononcée à partir de la valeur seuil de 0.25 ng et que le taux de germination
était de 92.25% et 95% pour le cas des variétés ELV, LBS, Syrie
2009-06-29T00:00:00ZAntagonism capability in vitro of Trichoderma harzianum against some pathogenic fungiHamitou, MokhtarDehimat, LAidhttp://depot.umc.edu.dz/handle/123456789/144382024-01-16T14:09:15Z2012-03-11T00:00:00ZAntagonism capability in vitro of Trichoderma harzianum against some pathogenic fungi
Hamitou, Mokhtar; Dehimat, LAid
The aim of this study is to clarify the antagonism capability in vitro of the antagonistic fungus
(Trichoderma harzianum) against the pathogenic fungus, four isolates of pathogenic fungus
associated internally with the solid wheat seeds ( Triticum durum )Desf , follower for species:
Botrytis cinerea, Cladosporium sp, Stemphylium botryosum and Alternaria sp, were brought. The
antagonistic sample (Trichoderma harzianum) was isolated from the wheat plant soil. The results
showed that:The direct confrontation of Trichoderma harzianum against the different fungus
isolates in vitro on PDA medium, showed in the third day of the experiment an inhibition in the
pathogenic mycelia growth, with a different ratios, it was equal to: 41.66% and 50% for
Stemphylium botryosum and Cladosporium sp, respectively, and amounted in the fourth day to
56.52% and 57.14% , for Botrytis cinerea and Alternaria sp, respectively, did not show any growth
of the different pathogenic fungi when re-planting a disk from the interaction hyphal areas
between the antagonistic fungus and the pathogenic fungus from the different dual cultures, while
the antagonistic fungus was grown. The microscopic observations of the different interactions
hyphal showed that the antagonistic fungus was affected on the pathogenic fungi with a several
biological forms: Decomposition phenomenon (Lyses): the antagonistic fungus was analyzed the
mycelia and spores of Cladosporium sp, while was analyzed the mycelia and damped the spore
formation in the other pathogenic fungi, compared with control. Parasitism phenomenon
(Mycoparasitism): it was found that the hyphae of Trichoderma harzianum has formed Haustoria
on the cell walls of Stemphylium botryosum hyphae and they penetrated within them. The
remote confrontation showed that the volatile metabolic substances of the antagonistic fungus
affected the growth of the pathogenic fungi , with a different rates over the seven days of
treatment, it peaked after two days of treatment to reach 13.33% and 50% in Botrytis cinirea
and Cladosporium sp, respectively, and decreased to 08.33% in the fourth day in Botrytis cinirea
and scored in the fifth day a ratio equal to 25.42% for Cladosporium sp, and was scored the
maximum ratio in the third day in Stemphylium botryosum to 23.07%,and lowered to 07.93% in
the seventh day, but in the Alternararia sp has recorded the lowest inhibition percentage to
05.55% in the third day and 05.76% in the seventh day. The microscopic observations Noted that
the volatile metabolic substances of the antagonistic fungus was affected the pathogenic fungi
with a several modes, their were with the mycelia analysis and prevent the spore formation in the
Alternararia sp, while was analyzed and aggregated the spores in the Cladosporium sp, and
stopped only the spore formation in both Botrytis cinirea and Stemphylium botryosum, compared
with control
2012-03-11T00:00:00Z