http://depot.umc.edu.dz/handle/123456789/4229 2026-06-01T17:39:24Z 2026-06-01T17:39:24Z Benkahoul, Malika Meraihi, Zahia Mechakra (Née Maza), Aicha http://depot.umc.edu.dz/handle/123456789/4646 2025-02-06T12:25:06Z 2002-02-20T00:00:00Z

Production de la protéase neutre par aspergillus oryzae sur déchets d'oranges. Optimisation du milieu de culture, purification partielle et étude des propriétés physico-chimiques de l'enzyme. Benkahoul, Malika; Meraihi, Zahia; Mechakra (Née Maza), Aicha 81 f.

2002-02-20T00:00:00Z HENNICHE SATOUF BOUSSEBOUA H. http://depot.umc.edu.dz/handle/123456789/4581 2022-12-27T17:33:02Z 1994-12-01T00:00:00Z

Influence de la découpe à chaud des carcasses de poulets de chair eviscérés sur leur qualité microbiologique HENNICHE SATOUF; BOUSSEBOUA H. 96 f.

1994-12-01T00:00:00Z MOKRANI DJAMILA DJEKOUN Abdelhamid. BENGUEDOUAR Ammar http://depot.umc.edu.dz/handle/123456789/4515 2022-12-27T22:22:12Z 2001-01-01T00:00:00Z

Etude de la symbiose; hedysarum coronarium L. rhizobium " hedysari " MOKRANI DJAMILA; DJEKOUN Abdelhamid.; BENGUEDOUAR Ammar Some strains of rhizobia were isolated from Hedysarum coronarium L. nodules, which was grown at the University campus of Constantine. The strains were selected after a cultural study.. The authentification of these strains allowed the identification of six isolates as Rhizobium hedysari high specific to Sulla nodulation. The development of the 6 strains, in the presence of the ones sp, on selective media classed those rhizobia as fast growing Rhizobium. ThePhysiological characterisation has shown that the optimal temperature is 30°C, the optimum pH is 6,8 and the development of the strains is possible up to 10% NaCl concentration. The numbers of rhizobiums per pelleted seeds, with the sterile peat-based inoculum, goes up to 106 rhizobiums par gram inoculants. The used techniques for bacteria identification (Intrinsic antibiotic resistance and phage sensitivity) permit identification of cultured isolates in Biskra region, due to the absence of specific strain in soil after the estimation of the number of rhizobia in soil (NPP test). The present study permitted the selection of resistance strains to saline stress and temperature ( maximum growth temperature is 40°C and salinity tolerance is inferior to 10%), also the possibility to extend the culture of Sulla in semi-arid area. 95 f.

2001-01-01T00:00:00Z Bennamoun ép Benmaiza Leila Meraihi Zahia http://depot.umc.edu.dz/handle/123456789/4497 2022-12-27T15:19:17Z 2001-09-30T00:00:00Z

Productionde l'a-amylase par aspergillus oryzae cultivé sur milieu à base de déchets d'oranges. Optimisation du milieu de culture, purification partielle et étude des propriétés physico-chimiques de l'enzyme Bennamoun ép Benmaiza Leila; Meraihi Zahia Orange pulp is a by-product of orange juice processing, contain a high levels of soluble sugars, was utilised as a basal medium at 2 % for a - amylase production by Aspergillus oryzae Ahlbtirg (Cohen) 1042.72. The selection of factors favoured a - amylase synthesis by the Plackett and Burman (1946) design and the determination of there optima by a central composite design of Box and Wilson (1951) allowed to retain soluble starch at 0.8 %, Yeast extract at 0.5 %, "corn steep liquor" at 1.98 %, magnesium sulphate at 0.2 g/l, manganese sulphate at 0.032 g/l, iron sulphate at 0.032 g/l and zinc sulphate at 0.009 g/l. The production of a - amylase is influenced by the type of carbon and nitrogen sources. Among the six carbon sources tested, starch is the best inducers resulted in 270% increase in the a - amylase productivity. However, among the fifth nitrogen sources tested "com steep liquor" is the best one, induce an increase in 188 % in a - amylase activity. The kinetics of growth and of the enzymatic production demonstrated that the a - amylase synthesis is of associated mycelial growth type, because the growth rate [ft = (1/x) (dx/dt)] and the production rate [e = (1/x) (dp/dt)] were positively correlated. The a - amylase was purified by sequential steps of ammonium sulphate, dialysis and Sephacryl S-200 gel filtration. By these steps, the purity of the enzyme increased by 18.50 and recovery of the enzyme activity was 43 %. The purification of the enzyme on Sephacryl S-200 allowed the separation of the enzyme into one molecular form. The purified enzyme had an optimal pH at 5, optimal temperature at 55°C. However, its half time was 90 minutes at 55°C and 40 minutes at 70°C. The Km and Vmax for starch were 4.25 mg/ml and 17.51 pmoles/min/mg of protein respectively. 120f.

2001-09-30T00:00:00Z