http://depot.umc.edu.dz/handle/123456789/14449 2026-06-02T15:45:30Z 2026-06-02T15:45:30Z ZATER, Zohra Yasmine MERZOUG, Mohamed ZERGUI, Amina http://depot.umc.edu.dz/handle/123456789/14700 2025-10-14T10:29:46Z 2023-10-05T00:00:00Z

AP-24: Characterization of the Trypsin Protease Gene from plantarum VF3 ZATER, Zohra Yasmine; MERZOUG, Mohamed; ZERGUI, Amina Trypsin protease is a crucial enzyme with diverse applications across various industries, including food, pharmaceuticals, and biotechnology. While extensively studied in eukaryotes, the trypsin from Lactobacillus plantarum VF3 presents intriguing differences in its structural and functional properties. Objectives: This study aims to identify and characterize the gene encoding trypsin protease in L. Plantarum VF3, highlighting its distinct enzymatic features. Methods: The L. plantarum VF3 strain, isolated from cow milk, was identified using the 16S rRNA gene method. Specific primers were employed for PCR analysis of the tlp gene, and the resulting products were sequenced using the BigDye™ Terminator v3.1 Cycle Sequencing Kit. The I-TASSER server was utilized for protein structure prediction and functional analysis. Results and discussion: The investigation confirmed the presence of the tlp gene within the genome of L. plantarum VF3, highlighting a notable difference in its amino acid sequence compared to human trypsin. This divergence in sequence correlates with distinct catalytic properties, where lactobacillus trypsin displays enhanced specificity for substrates. These findings emphasize the distinctiveness of L. plantarum VF3 trypsin in its potential applications within the biotechnology and food industries, paving the way for novel enzymatic solutions with tailored properties. Conclusion: This study elucidates trypsin protease's genetic basis and distinctive characteristics in L. plantarum VF3. The observed differences in sequence and catalytic properties compared to eukaryotic trypsin open exciting possibilities for exploiting this enzyme in various industrial applications, ranging from food processing to biotechnology. Further research in harnessing these unique features promises innovative solutions in enzyme technology. Keywords: Trypsin, Lactobacillus plantarum, 16S rRNA, PCR, I-TASSER, enzyme technology

2023-10-05T00:00:00Z YAHIAOUI, Younes NETTOUR, Djamel MERCHICHI, Amira http://depot.umc.edu.dz/handle/123456789/14699 2025-10-14T10:21:43Z 2023-10-05T00:00:00Z

AP-23: Caractérisation environnementale des métaux lourds issus des rejets phosphatiers de Djbel Onek, Tébessa – Algérie YAHIAOUI, Younes; NETTOUR, Djamel; MERCHICHI, Amira Dans l’industrie minière, tous les procédés de séparation et d’enrichissement générent non seulement un concentré riche de l’espèce minérale, mais aussi une quantité importante des rejets sous forme de liquide où de solide. Leur stockage à l’air libre dans des parcs à résidus peuvent provoquer plusieurs impacts environnementaux. Les métaux lourds sont les micropolluants métalliques qui peuvent contaminer l’environnement. Ces derniers constituent un problème préoccupant lorsqu’ils sont impliqués dans la pollution de l’eau et le sol, leur toxicité peut être forte dommageable pour le milieu, mais leur accumulation au fil de la chaîne alimentaire pourrait avoir des effets néfastes sur la santé humaine, la faune et la flore. Notre travail porte sur la caractérisation des rejets phosphatiers de Djbel Onek, Tébessa – Algérie qui confirme une contamination des sols et des plantes de la région essentiellement par le Pb, Zn et Cd. L’évaluation des risques liés aux métaux lourds a montré que les phosphates bruts et leurs rejets miniers produisent des éléments traces métalliques ETM dangereux dépassant les normes pour les sols (par exemple, U, Cd, Cr, Mo, V et Tl). Par conséquent, en raison de l’exposition aux rejets miniers et de la mobilisation des particules les plus fines par le vent et les pluies, ces métaux dangereux pourraient avoir des effets néfastes sur l’environnement et la santé humaine

2023-10-05T00:00:00Z TENIOU, Ahlem ZERMANE, Marwa MADI, Ibrahim A MOUHOUB, Riane MARTY, Jean Louis RHOUATI, Amina http://depot.umc.edu.dz/handle/123456789/14698 2025-10-14T10:07:04Z 2023-10-05T00:00:00Z

AP-22: One-Step Chemiluminescent Assay for Hydrogen Peroxide Analysis in Water TENIOU, Ahlem; ZERMANE, Marwa; MADI, Ibrahim A; MOUHOUB, Riane; MARTY, Jean Louis; RHOUATI, Amina In this work, we aim to develop a simple, and rapid, nonenzymatic and homogeneous biosensor for sensitive and rapid quantification of hydrogen peroxide. Methods: In this technique, hemoglobin was used as a bioreceptor, where heme groups act as electroactive centers to catalyze hydrogen peroxide reduction. The chemiluminescence reagent; luminol is also a peroxidase substrate and can be oxidized by hemoglobin thus generating a CL signal. The working principle was based on the competition between hydrogen peroxide and luminol towards hemoglobin. A 96-well microplate was used to perform this assay. For thatat, 25 µL of Hb solution (3 µg mL−1) was added to each well, followed by 25 µL of different concentrations of H2O2. After incubation, 25 µL of luminol solution was also added. Then, the chemiluminescence intensity was measured at an emission wavelength of 425 nm. Results and discussion: The detection principle is mainly based on the chemiluminescence signal diminution in the presence of H2O2. Because this latter will react with Hb that is essential for luminol reaction, thus leading to a lower chemiluminescence signal. AlsoUnder optimized conditions, the chemiluminescent signal decreased with increasing hydrogen peroxide concentrations within the linear range of 0.5 to 12 mM, with a correlation coefficient R2 of 0.99762. The limit of detection was calculated to be as low as 0.308 mM. The selectivity of the biosensor was successfully demonstrated against different interferents. Conclusion: In this work, an homogenous chemiluminescent assay was developed using Hb as a bioreceptor. The developed strategy provides a one step, simple, and low-cost bioanalytical method which can be applied for the monitoring of hydrogen peroxide

2023-10-05T00:00:00Z TALHI, Lina SEBAIHIA, Mohammed http://depot.umc.edu.dz/handle/123456789/14697 2025-10-14T09:46:25Z 2023-10-05T00:00:00Z

AP-21: The Crucial Importance of Rapid and Effective Fire Blight Detection TALHI, Lina; SEBAIHIA, Mohammed Fire blight is a highly contagious bacterial disease that poses a significant threat to Algeria's agricultural sector, particularly fruit trees like apples and pears. unchecked spread of fire blight can devastate entire orchards and spread from one orchard to another, potentially causing regional epidemics with far-reaching consequences for the national economy. Moreover, unchecked fire blight often leads to indiscriminate use of antibiotics and harmful chemical treatments, negatively impacting the environment and human health. Effective and rapid detection methods of infected trees is crucial to minimize economic losses and protect the livelihoods of countless fruit cultivators and the entire agricultural sector and aligning with Algeria's commitment to sustainable agriculture and providing a proactive solution to this contagious bacterial threat. To achieve rapid and effective fire blight detection in Algeria's agricultural sector, substantial investments in cutting-edge technologies are necessary. Here we discuss Modern diagnostic tools such as PCR tests which can significantly expedite the identification of infected trees. Rapid diagnostic tests enable the quick identification of fire blight bacteria or their genetic material in plant samples. Once detected, farmers and agricultural authorities can take timely action to quarantine infected plants, apply appropriate treatments, and implement control measures to prevent further dissemination. In conclusion, the vital importance of rapid and effective fire blight detection in Algeria's agricultural sector cannot be overstated. It impacts the economy, food security, and sustainability of the nation's agricultural industry

2023-10-05T00:00:00Z