Thème 02: Valorisation des ressources naturelles

The valorization of bioactive compounds through phytochemical investigation, antioxidant activity and histo-anatomical study of a medicinal plant from Mila city, Algeria (Ecbalium elaterium L.)

2023-10-23T08:07:06Z

The valorization of bioactive compounds through phytochemical investigation, antioxidant activity and histo-anatomical study of a medicinal plant from Mila city, Algeria (Ecbalium elaterium L.) CHAHNA, Renda; SADOU, Nina; BARKAT, Ramzi; SERIDI, Ratiba The aim of this work is the study of new biologically active agents, derived from an endemic medicinal plant widely used by the Algerian population, which is Ecbalium elaterium. Our study is composed of two complementary axes, namely a botanical study and a phytochemical study of the leaves of this species. Phytochemical screening was carried out by specific coloring and precipitation reactions. The results of the powder macerate and the extract of aerial parts of Ecbalium elaterium L. showed the presence of various bioactive compounds such as terpenoids, steroids, tannins, reducing compounds, flavonoids, etc. On the other hand, the absence of saponins, leucoanthocyanes, and, anthraquinones was noticed. The quantification of total antioxidant capacity was evaluated through the study of the antioxidant activity of the plant ethanolic extract using DPPH radical scavenging technique. The botanical study was made by a macroscopic and microscopic analysis of the vegetative organs of Ecballium elaterium L. by the analysis of vegetal material, both integral and as powder using optical microscopy. Histo-anatomical details were highlighted by coloration with an original combination of reagents for the double coloration of cellulose and lignin using Congo red and methyl green reagents. The botanical analysis revealed all the macroscopic and microscopic characteristics which allow the botanical identification of Ecbalium elaterium L. However, further, detailed studies are urgently needed to check the therapeutic potentiality, safety, and mechanism of action of Ecbalium elaterium L. In perspective, we plan to deepen the study of this species using innovative extraction methods

Antioxidant and antibacterial activities of bulb extracts from Scilla maritime

2023-10-23T07:56:15Z

Antioxidant and antibacterial activities of bulb extracts from Scilla maritime Saffidine, Karima; Zerargui, Fatima; Guemmaz, Thoraya; Laroui, Haifa; Baghiani, Abderahmane Scilla maritima is widely used for the treatment of dermatological problems, respiratory and vaginal ailments, in the region of Setif. The present study reports the evaluation of antioxidant, antihemolytic and antibacterial activities of bulb extracts (CrE: crude extract, HeE: hexane fraction, EaE: ethyl acetate fraction and AqE: aqueous fraction) from this medicinal plant. Total polyphenols and flavonoids of different extracts were determined and ranged from 60.7 to 168.72 ± 5.89 EAG/g and 5.93 to 11.68 µg EQ/mg, respectively. The antioxidant activity of these extracts was evaluated by several methods. Potent DPPH scavenging activity was exhibited by EaE and AqE (IC50 of 24.77±0.96 and 46.93±1.44 µg/ml), and these fractions were either the most active in inhibition of β-carotene oxidation (86.38 and 92.36%). CrE showed a significant chelating activity (IC50 = 553.07±51.14 µg/ml). While, EaE displayed the greater reducing power (EC50 = 38.5±1.14 µg/ml) and inhibited significantly the hemolysis of red blood cells. Antibacterial activity was performed using the disc diffusion method and the inhibition zones varied from 11.5 to 33 mm. On the other hand, MIC and MBC ranged from 0.09 to 6.25 mg/ml and 1.56 to 25 mg/ml, respectively. Results indicate that CrE was bactericidal against Bacillus cereus, but bacteriostatic against Staphylococcus aureus and Proteus mirabilis. However, EaE was bactericidal against these bacterial strains. In addition, good synergistic effects were obtained between extracts and standard antibiotics, where inhibition zones varied between 32 and 48 mm. hese results support the use of this species in traditional medicine and could be used where antioxidant and antibacterial are warranted

Antihemolytic, Antithrombotic effects of five flavonoids compounds isolated from Jordanian Varthemia iphionoids

2023-10-23T07:44:32Z

Antihemolytic, Antithrombotic effects of five flavonoids compounds isolated from Jordanian Varthemia iphionoids Zerargui, F; Saffidine, K; Guemmaz, T; Laroui, H; Baghiani, A The aim of this study is to evaluate in-vitro, the biological activities of some flavonoïds isolated from Varthemia iphionoids (Asteraceae family) aqueous methanol extract. The plant is a perennial herb, common in mountainous places in many parts of Jordan. The plant is described in Jordanian folk medicine for the treatment of kidney and gastro-intestinal disorders. V. iphionoids aqueous methanolic extract afforded sixteen known compounds (three monoterpenoids, twelve flavonoids and one sterol). The antioxidant activities of five identified flavonoids: kumatakillin (VA1), Penduletin (VA2), Jeceidine (VA3), 6- methoxyisokaemfride (VA4) and quercetin 3,3’-Di-O-methyl ether (VA5) were determined. The results indicated that in anti-hemolytic assay, the five compounds gave a TH50 higher than standard and the best result was shown with VA2 (TH50 = 60.14 ± 0.72). Moreover, the evaluation of anticoagulant and thrombolytic potential showed that all the compounds had significantly prolonged the clotting time and exhibited an important thrombolytic activity especially the compounds VA1, VA2 and VA3 compared to the control (*** p <0.001)

Antioxidants in plants as a valorization pattern emphasizing the need for the Conservation of Plant Biodiversity: Algeria as a case

2023-10-23T07:35:15Z

Antioxidants in plants as a valorization pattern emphasizing the need for the Conservation of Plant Biodiversity: Algeria as a case RAMLI, Imane Plants are phytochemical hubs containing antioxidants, essential for normal plant functioning and adaptation to environmental cues and delivering beneficial properties for human health. Therefore, knowledge of the antioxidant potential of different plant species and their nutraceutical and pharmaceutical properties is of utmost importance. Exploring this scientific research field provides fundamental clues on (1) plant stress responses and their adaptive evolution to harsh environmental conditions and (2) (new) natural antioxidants with a functional versatility to prevent and treat human pathologies. Algeria is a massive country with a wide variety of landscapes, ecological zones, nature reserves, and national parks. It is particularly considered by many to be the most unique natural country in the Mediterranean with rich plant biodiversity harboring a great antioxidant potential. However, the biodiversity of these plants is largely unknown and therefore underexploited. This natural antioxidant reservoir can be valorized via plant-derived foods and products in local areas. Besides opening new avenues for the implementation of sustainable agroecological practices in crop production, it will also contribute to new strategies to preserve plant biodiversity and simultaneously improve nature management policies in Algeria. This study provides an overview of the beneficial properties of antioxidants for plant protection and human health and is directed to the valorization of these plant-derived antioxidants, emphasizing the need for biodiversity conservation practices in Algeria