http://depot.umc.edu.dz/handle/123456789/12843 2026-05-03T09:26:05Z 2026-05-03T09:26:05Z ZITOUNI, Hind CHERFIA, Radia GHORRI, Sana CHADI, Oumnia BELGHIT, Hadjer http://depot.umc.edu.dz/handle/123456789/14804 2026-01-18T12:54:56Z 2023-10-05T00:00:00Z

HP-50: Alternative Therapeutic Strategy to Antibiotics for the Treatment of Urinary Tract Infection ZITOUNI, Hind; CHERFIA, Radia; GHORRI, Sana; CHADI, Oumnia; BELGHIT, Hadjer Essential oils (EOs) are currently being recognized for enhancing the quality of life and alleviating a variety of illnesses. Multiple antibacterial properties of plants that contribute in suppressing uropathogenic invasion are one of the main strategies to decrease or eliminate bacterial progression. Objectives: The objectives of this investigation were the extraction and the study of the antimicrobial activity of the EOs from plants against uropathogenic bacteria, in orther to evaluate their potential as alternative products of synthetic antibiotics. quantitatively assessed. Methods: EOs from Petroselinum crispum, Thymus vulgaris and Cinnamomum cassia were obtained by hydrodistillation method. Where their antibacterial activity against uropathogenic bacteria; Escherichia coli, Staphylococcus sp, Pseudomonas sp and Klebsiella sp were qualitatively and Results and discussion: The yields of the three EOs are respectively 0.49% for parsley, 2.25% for thyme, and 2.35% for cinnamon. The tested EOs exhibit an interesting antibacterial activity compared with that of the antibiotics; cotrimoxazole, fluoroquinolones, and β-lactams. Cinnamon and thyme oils have a good activity against Escherichia coli, Staphylococcus sp, and Klebsiella sp, but a moderate activity against Pseudomonas sp. However, parsley oil showed a weak antibacterial activity. Conclusion: The increasing rates of uropathogens resistance against the tested antibiotics which cannot be used for empiric therapy. For that, to counteract the increasing resistance rates, alternative treatment options, such as phytotherapeutic approaches, need to be investigated

2023-10-05T00:00:00Z ZERMANE, Maroua TENIOU, Ahlem RHOUATI, Amina BERKANI, Mohamed http://depot.umc.edu.dz/handle/123456789/14802 2026-01-18T10:07:12Z 2023-10-01T00:00:00Z

HP-49: A Simple Fluorescent Aptasensor Based on MXene for Amoxicillin detection ZERMANE, Maroua; TENIOU, Ahlem; RHOUATI, Amina; BERKANI, Mohamed In this study, our focus was on the development of a sensitive and a selective biosensor based on FAM labelled aptemer and FRET process using 2D nanosheet MXene as a quencher of fluorescence for amoxicillin detection. Objectives: The use of amoxicillin in human and veterinary medicine can result in the appearance of residues in food and the aquatic environment, which poses undesirable health risks for consumers. To help overcome this problem, it may be advantageous to detect and measure the levels of amoxicillin residues. Therefore, we propose a simple, sensitive and selective aptasensor for amoxicillin detection. Methods: to realize the fluorescent aptasensor, MXene was used as agent of fluorescence quenching. Firstly, the MXene concentration and quenching time were optimized. Than the detection of amoxicillin was realized in a 96-well black microplate, for which an appropriate volume of FAM Apt was incubated with different concentrations of AMOX, then MXene solution was added to the mixture and stirred. Finally, the fluorescence intensity was measured. Results and discussion: Fluorescence measurements showed a wide linear range from 100 to 2400 ng/ml and a low detection limit of 1.53 ng/ml witch was lower than those reported amoxicillin detection assays. The selectivity of this detection system was tested and the result confirms that the developed aptasensor has excellent specificity for the detection of AMOX, indicating its potential application in complex matrices. Conclusion: The developed aptasensor is excellent for the detection of AMOX, this detection system based on two-dimensional MXene nanosheets, has the potential to be expanded in order to target other antibiotics. Thus widening the method's scope and offering potential for more comprehensive monitoring of various antibiotics

2023-10-01T00:00:00Z ZERGUI, Amina ZATER, asmine http://depot.umc.edu.dz/handle/123456789/14800 2026-01-18T09:49:55Z 2023-10-05T00:00:00Z

HP-48: Probiotics and Multi-Drug Resistant Urinary Tract Infection ZERGUI, Amina; ZATER, asmine Probiotics are beneficial microorganisms that can have a positive impact on gut health and potentially influence various aspects of the body's immune and inflammatory responses. Multi-drug resistant urinary tract infections (MDR-UTIs) are a concerning health issue, as they are more challenging to treat due to the limited effectiveness of antibiotics. Probiotics have been studied for their potential role in preventing and managing UTIs, including MDR-UTIs, although the research in this area is still evolving Objectives: This study was performed to determine the prevalence of uropathogens causing urinary tract infections (UTIs), their pattern of antimicrobial resistance and evaluating the recurrence of urinary tract infections after taking probiotics Methods: This research was conducted in Oran and involved the examination of 182 urine specimens, of patients suffering from recurrent urinary tract infections, obtained from a private laboratory. Fully automated VITEK 2 compact system was used in the identification and antimicrobial susceptibility testing of causative microorganisms. To assess the recurrence of these infections, 91 patients took oral probiotics for one month, and the others did not. Results and discussion: Antimicrobial resistance was seen both in Gram-positive and Gram negative bacteria. Multiple resistances were high among the isolated urinary pathogens. Particularly, E. coli, Enterobacter cloacae and Staphylococcus aureus, which had very high resistance rate to the tested antimicrobial agents. The recurrence of urinary tract infections in patients who took probiotics in addition to antibiotics decreased by 40%, unlike those who only took antibiotics. Conclusion: Although prophylactic antibiotics remain the preferred preventive treatment in recurrent UTIs, the emergence of antimicrobial resistance worldwide has made the development of non antibiotics strategies a priority. The idea is that probiotics may help maintain a balanced and healthy urinary tract microbiota, making it less susceptible to infection

2023-10-05T00:00:00Z YOUCEF-ALI, Mounia Edward MAKONGE, Flora KACEM CHAOUECHE, Noreddine http://depot.umc.edu.dz/handle/123456789/14799 2026-01-18T09:39:19Z 2023-10-01T00:00:00Z

HP-47: Optimization of protease production from an antifungal Bacillus subtilis under different culture conditions YOUCEF-ALI, Mounia; Edward MAKONGE, Flora; KACEM CHAOUECHE, Noreddine Protease is one of the enzymes which find wide industrial and pharmaceutical applications. Objectives: The soil bacterium Bacillus subtilis (accession number KC341751) was investigated in the optimization of protease production under different culture conditions. This strain was first, screened and evaluated for its antifungal activity against Candida albicans, one of the most important human fungal pathogens. Methods: The capacity of B. subtilis to produce protease was shown by In vitro assay with the antagonist and its cell-free culture supernatant, on agar plates. The identification of this strain was then done based on its morphological as well as biochemical characteristics, later confirmed by 16s rDNA sequencing and BLAST analysis. Besides, the screening of protease activity by B. subtilis was checked by growing it on specific agar medium; by spot inoculation method. Moreover, in order to optimize the production of this enzyme, B. subtilis was grown under different values of pH, Temperature, and diverse concentrations of glucose. Results and discussion: The results revealed that B. subtilis inhibited the growth of C. albicans, by developing a hydrolysis zone of 25 mm. Furthermore, the screening of protease activity showed a high zone of activity (34 mm) after 24h of incubation at 30°C. In addition, the optimization of the protease production under various conditions exhibited that the optimal pH and temperature were 7 and 50°C, with a proteolytic activity of 45.24U/ ml and 17.86U/ml respectively. However, the cultivation of B. Subtilis on different concentrations of glucose showed that the proteolytic activity increases as a function of the increase of the glucose concentration in the culture medium namely; 13.69U/ml for 0.4% glucose. Conclusion: These results express that the protease produced in the present study is neutral and thermostable. The presence of glucose in the medium with pH 7 and temperature 50°C enhanced enzyme production. Thus, such conditions were able to acts as a promoter for better protease activity; wich is very interesting in various industries especially pharmaceutical one

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